Laboratory of Plant Cell Biology

TAKAGI, Shingo
mail shingot @
Assistant Professor
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Plant Science


Graduate School of Science



Research Theme

When you study functions of biological systems, you may have two types of questions, namely, ‘Why questions’ that ask the reason and significance of their functions, and ‘How questions’ that ask the mechanism responsible for their functions. Basically, ecologists ask ‘Why questions’ while physiologists ask ‘How questions’. However, scientists in both groups have never been always satisfied with their own ways. To develop the frontiers of plant ecology, morphology, and physiology, we are asking ‘How questions’ on the ecological phenomena and ‘Why questions’ on the physiological phenomena.

Cellular responses to environmental fluctuations

Although plants cannot change their locations, they sense and respond to various changes in environmental factors quite precisely. Using cell biological techniques, we are dissecting such processes as chloroplast movement, tropic curvature, and cytoskeletal organizations.

Ecophysiological studies on photosynthesis

Plants show maximal production of photosynthesis using limited environmental resources, such as light intensity, nitrogen. We are studying about mechanisms of photosynthetic systems on one leaf and whole-plant levels. We also examine effects of growth temperature and virus infection on photosynthesis. Recently, we found that CO2 can be transported via aquaporin on cell membrane in leaves.

0Chloroplasts face with the intercellular spaces (modified from J. Exp. Biol. 206:1963-1969, 2003). A: Cross section of the adaxial part of a Spinacia oleracea (spinach) leaf. B: Paradermal section of the parenchyma cells of Maesa japonica. Arrowhead in A indicates the stoma and the orange circles in B indicate intercellular spaces. Bars: 25 µm.


Islam M.S., Van Nguyen T., Sakamoto W., Takagi S. Phototropin‐ and photosynthesis‐dependent mitochondrial positioning in Arabidopsis thaliana mesophyll cells. Journal of Integrative Plant Biology 62(9) , 1352 - 1371 (2020)

Harada A., Okazaki Y., Kinoshita T., Nagai R., Takagi S. Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in Vallisneria Mesophyll Cells. Plants 9(3), 376 , (2020)

Iida M., Takano T., Matsuura T., Mori IC, Takagi S. Circumnutation and distribution of phytohormones in Vigna angularis epicotyls. Journal of Plant Research 131(1) , 165 - 178 (2018)

Sakai Y., Inoue S., Harada A., Shimazaki K., Takagi S. Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells. Journal of Integrative Plant Biology 57 , 93 - 105 (2015)

Sakamoto Y., Takagi S. LITTLE NUCLEI 1 and 4 regulate nuclear morphology in Arabidopsis thaliana. Plant and Cell Physiology 54 , 622 - 633 (2013)

Takamatsu H., Takagi S. Actin-dependent chloroplast anchoring is regulated by Ca2+-calmodulin in spinach mesophyll cells. Plant and Cell Physiology 52(11) , 1973 - 1982 (2011)

Iwabuchi K., Minamino R., Takagi S. Actin reorganization underlies phototropin-dependent positioning of nuclei in Arabidopsis thaliana leaf cells. Plant Physiology 152 , 1309 - 1319 (2010)

Takagi S., Islam M.S., Iwabuchi K. Dynamic behavior of double-membrane-bound organelles in plant cells. International Review of Cell and Molecular Biology 286 , 181 - 222 (2011)


TEL:06-6850-5818 FAX:06-6850-5818