Laboratory of Single Molecule Biology


Masahiro Ueda
Assistant Professor
Yukihiro Miyanaga

Signal Transduction Biology


Graduate School of Science, Department of Biological Sciences


Toyonaka campas

Research Theme

Cells are complex but well-organized systems comprising various kinds of biomolecules. Because biomolecules operate stochastically under the strong influence of thermal fluctuations, living cells can be referred to as stochastically-operating biomolecular computation systems. Through the dynamic processes in reaction networks of biomolecules, cells can respond flexibly and adaptively to environmental changes. Recent progress in single molecule imaging techniques has made it possible to monitor directly the stochastic behaviors of biomolecules in living cells, in which the locations, movements, turnovers, and complex formations of biomolecules can be detect ed quantitatively at the single molecule level, providing powerful tools to elucidate molecular mechanisms of intracellular signaling processes. Our laboratory develops quantitative single-molecule imaging methods, computational modeling methods and biochemical synthetic methods to reveal the molecular mechanisms of cellular chemotaxis with single-molecule resolution.

Development of automated in-cell single-molecule imaging system (AISIS)

Single-molecule biology of chemotactic signaling system

Synthetic biology of chemotactic signaling system


Okamoto, M., Namba, T., Shinoda, T., Kondo, T., Watanabe, T., Inoue, Y., Takeuchi, K., Enomoto, Y., Ota, K., Oda, K., Wada, Y., Sagou, K., Saito, K., Sakakibara, A., Kawaguchi, A., Nakajima, K., Adachi, T. Fujimori, T., Ueda, M., Hayashi, S., Kaibuchi, K. and Miyata, T. TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding. Nature Neuroscience doi:10.1038/nn.3525 , (2013)

Matsuoka, S., Shibata, T. and Ueda, M. Asymmetric PTEN distribution regulated by spatial heterogeneity in membrane-binding state transitions. PLoS Computational Biology 9 , e1002862 - (2013)

Tsujioka, M. Yumura, S., Inouye, K., Patel, H., Ueda, M. and Yonemura, S. Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis. Proc. Natl. Acad. Sci. USA. 109 , 12992 - 12997 (2012)

Nishimura, S. I., Ueda, M. and Sasai, M. Non-Brownian dynamics and strategy of amoeboid cell locomotion Physical Review E. 85 , 041909 - (2012)

Arai Y., Shibata T., Matsuoka S., Sato M. J., Yanagida T. and Ueda M. Self-organization of the phosphatidylinositol lipids signaling for random cell migration. Proc. Natl. Acad. Sci. USA. 107 , 12399 - 12404 (2010)

Matsuoka S., Shibata T. and Ueda M. Statistical analysis of lateral diffusion and multi-state kinetics in single-molecule imaging. Biophys. J. 97 , 1115 - 1124 (2009)

Sato M. J., Kuwayama H., van Egmond W. N., Takayama A. L. K., Takagi H., van Haastert P. J. M., Yanagida T. and Ueda M. Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling. Proc. Natl. Acad. Sci. USA. 106 , 6667 - 6672 (2009)

Ueda M. and Shibata T. Stochastic signal processing and transduction in chemotactic response of eukaryotic cells. Biophys. J. 93 , 11 - 20 (2007)

Matsuoka, S., Iijima, M., Watanabe, T. M., Kuwayama, H. Yanagida, T., Devreotes, P. and Ueda M. Single molecule analysis of chemoattractant-stimulated membrane recruitment of a PH domain-containing protein. J. Cell Sci. 119 , 1071 - 1079 (2006)

Vazquez, F., Matsuoka, S., Sellers, W. R., Yanagida, T., Ueda, M. and Devreotes, P. Tumor suppressor PTEN acts through dynamic interaction with the plasma membrane. Proc. Natl. Acad. Sci. USA. 103 , 3633 - 3638 (2006)


Graduate School of Science, Osaka University

Machikaneyama, Toyonaka
Osaka 560-0043,