Membrane Protein Chemistry Laboratory

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Associate Professor
Joji MIMA
mail Joji.Mima@protein.osaka-u.ac.jp
Fields

Functional Biomolecular Biology

Belong

Institute for Protein Research

Location

Suita

Research Theme

Molecular basis of intracellular membrane dynamics: membrane fusion, fission, and deformation.

Understanding intracellular membrane fusion by cell-free reconstitution with defined components

Intracellular membrane fusion is a fundamental and conserved biological reaction which is vital for vesicle trafficking between cellular compartments, organelle morphology, hormone secretion, and neurotransmission. Fusion is regulated by specific proteins and lipids: SNAREs, SNARE chaperones, Rab GTPases, and phopsphoinositides. Though these proteins and lipids cooperate for the assembly of each component into fusion-competent membrane microdomains, it is unclear how they act together to drive membrane fusion. In our group, we explore this vital membrane fusion machinery in eukaryotic cells by reconstituting proteoliposomal fusion with purified components and have been revealing that the ternary synergy of two SNARE chaperone systems and phosphoinositides is essential for fusion and that the synergy catalyzes the SNARE complex assembly and remodels the assembled SNARE complexes to trigger rapid membrane fusion.

Bibliography

Contact

Institute for Protein Research, Osaka University
3-2 Yamadaoka, Suita, Osaka 565-0871, Japan

http://www.protein.osaka-u.ac.jp/icr/mima/index.html

http://www.protein.osaka-u.ac.jp/icr/mima/index.html